Expression of Heat Shock Protein HSP90 in Genomic-DNA of Chickpea (Cicer arietinum L.) Callus by Heat Shock Treatment
Authors: Anwaar F AL-Taee (1*), Dr. Jamella H Rasheed (2)
Ph.D. Student, Department of Biology, Education College for Pure Sciences, University of Mosul, Iraq (1*)
Prof. Doctor in Biology, Department of Biology, Education College for Pure Sciences, University of Mosul, Iraq (2)
*Email: anwar.altaee@uomosul.edu.iq
Doi: doi.org/10.52132/Ajrsp.e.2022.33.6
This study was able to detect of the expression activity of heat shock proteins HSP90 and
heat transcription factors HSFs for the first time in callus cultures of chickpea, Cicer
arietinum L., that exposed to abiotic shocks, grown on MS medium supplemented with 1.0 mg L-1
naphthalene acetic acid (NAA) and 2.0 mg L-1 benzyl adenine (BA). Heat shock proteins HSPs were
constructed for increase of withstand long-term physical shocks, and production of resistant to
heat chickpeas plants, this shock was enhancement of tolerance of chickpea callus to abiotic
stresses (high - temperatures). Results enhanced the ability of chickpea callus to abiotic stresses
bearing and induce of HSF genes to heat shock proteins HSP90 production quickly to removing denatured
proteins, avoid apoptosis, thus, supporting tolerance to the sudden action of these shocks. Expression
activity of heat shock genes and transcription factors by determined based on polymerase chain reaction
qPCR, that explained the gene activity increasing at shocks intensity increased
Chickpea (Cicer arietinum L.), Heat stress, Heat shock protein HSP90, RNA sequencing, gene Expression
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